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KMID : 0377519820070030223
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Chung-Ang Journal of Medicine
1982 Volume.7 No. 3 p.223 ~ p.231
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Studies on the Activities of Isocitrate Dehydrogenase in the Red Blood Cells of Normal and PhenyIhydrazine-treated Rabbits
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Chung Dong-Chull
Kwon Nyoun-Soo
Lee Dong-Wook
Lee Hi-Sung
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Abstract
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In order to establish a general pattern of synthesis and degradation of cytosolic and mitochondrial isocitrate dehydrogemase isozymes in the red blood cells of normal and phenlydeazine treated rabbits were conducted in this study. The activity of isocitrate dehydrogemase was measured by the method of King. The results and summarized as follows: 1. The reticulocyte count of normal rabbit blood was approximately 2.5%, and the administration of phenlydeazine resulted in rapid and profound reticlocytosis, i.e., about 63.5% on dat essentially 100% on day 7. 2. Total activity of isocitrate dehydrogenase in normal blood cells was 0.95 unit/ml RBC and the relative activities of cytosolic and mitochondrial isocitrate dehydeogenase isozymes were 91.6% and 8,4%, respectively. 3. In phenlydeazine treated rabbits, total isocitrate dehydrogenase activity was maximal on day 8, about 2.57 unit/ml RBC, which is about 2.7 fold higher than normal value. The relative activities of cytosolic and mitochondrial isocitrate dehydrogenase were 56.8% and 43.2%, respectively. 4. The increase in total isocitrate dehydrogenase activity during the induction of reticulocytisis appeared to be primarily due to 14.3 fold increase in the activity of the mitochondrial isocitrate dehydrogenase isozyme(Ks=0.0152; Kd=0.0133; t1/2=52.11 hrs). 5. The activity of the cytosolic isocitrate dehydrogenase isozyme in phenylhydrazine treated rabbit red blood cells increase to about 1.7 times its normal value(Ks=0.0102;Kd=0.0070; t1/2=99.00 hrs). 6. The rate constants following recovery from phenylhydrazine treatment were: Ks=0.0008; Kd=0.0104;t1/2=66.63 hrs for the mitochondrial isozyme and Ks=0.0208; Kd=0.0239; t1/2=29.00 hrs for the cytosolic isozyme.
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